duoset enzyme linked immunosorbent assay kits  (R&D Systems)

Journal: ACS Nano

Article Title: An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain

doi: 10.1021/acsnano.3c08097

Figure Lengend Snippet: Validation of adsorption capacity of nanomaterials with different concentration gradients on inflammatory factors/LPS/NGF. The remaining concentrations of TNF-α (A), IL-1β (B), IL-6 (C), IFN-γ (D), LPS (E), and NGF (F) were detected by an enzyme-linked immunosorbent assay (ELISA), after coculturing with MnO 2 nanoparticles, MnO 2 @MNP nanoparticles, and MnO 2 @TMNP nanoparticles at different concentrations. The initial concentrations of cytokines, LPS and NGF were 500 pg/mL. Data are presented as the mean ± SD ( n = 3): ns, not significant; ****, p < 0.0001 between groups.

Article Snippet: The cytokines used in this study (TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)), lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress (MCE, China).

Techniques: Biomarker Discovery, Adsorption, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: ACS Nano

Article Title: An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain

doi: 10.1021/acsnano.3c08097

Figure Lengend Snippet: Validation of the adsorption capacity of fixed concentrations of nanomaterials to inflammatory cytokines/LPS/NGF with different concentration gradients. The remaining concentrations of TNF-α (A), IL-1β (B), IL-6 (C), IFN-γ (D), LPS (E), and NGF (F) at different initial concentrations were detected, after coculturing with 50 μg/mL MnO 2 @TMNP. Data are presented as the mean ± SD ( n = 3): ns, not significant; ****, p < 0.0001 between groups.

Article Snippet: The cytokines used in this study (TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)), lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress (MCE, China).

Techniques: Biomarker Discovery, Adsorption, Concentration Assay

Journal: ACS Nano

Article Title: An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain

doi: 10.1021/acsnano.3c08097

Figure Lengend Snippet: MnO 2 @TMNP inhibits the LPS-induced M1 polarization of macrophages. (A) Schematic diagram of the experimental design. (B) Flow cytometry detecting F4/80+CD86+ cells and F4/80+CD206+ cells to evaluate the polarization of macrophages. (C) Quantification of the CD86 geomean fluorescence intensity of macrophages according to flow cytometry. (D) Quantification of the proportion of M1 macrophages in each group. The levels of mRNA encoding iNOS (E), TNF-α (F), and IL-6 (G) in macrophages treated with LPS, LPS+MnO 2 , LPS+TMNP, or LPS+MnO 2 @TMNP, respectively. The fold change was normalized to the control group. The concentrations of TNF-α (H), IL-1β (I), IL-6 (J), and IFN-γ (K) in the supernatant after treating macrophages with LPS, LPS+MnO 2 , LPS+TMNP, or LPS+MnO 2 @TMNP. Data are presented as the mean ± SD ( n = 3): ns, not significant; ****, p < 0.0001 between groups.

Article Snippet: The cytokines used in this study (TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)), lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress (MCE, China).

Techniques: Flow Cytometry, Fluorescence, Control

Journal: ACS Nano

Article Title: An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain

doi: 10.1021/acsnano.3c08097

Figure Lengend Snippet: MnO 2 @TMNP inhibits H 2 O 2 -induced M1 macrophage polarization. (A) Schematic illustration of the establishment of H 2 O 2 -induced macrophage M1 polarization to assess the effects of MnO 2 @TMNP on alleviating the inflammatory microenvironment. (B) Flow cytometry of macrophage polarization after treatment with H 2 O 2 , H 2 O 2 +MnO 2 , H 2 O 2 +TMNP, or H 2 O 2 +MnO 2 @TMNP. (C) CD86 geomean fluorescence intensity of macrophages according to flow cytometry. (D) Quantification of the proportion of M1 macrophages in each group. The mRNA content of TNF-α (E), iNOS (F), and IL-6 (G) in macrophages treated with H 2 O 2 , H 2 O 2 +MnO 2 , H 2 O 2 +TMNP, or H 2 O 2 +MnO 2 @TMNP. The fold change was normalized to the control group. The concentrations of TNF-α (H), IL-1β (I), IL-6 (J), and IFN-γ (K) in the supernatant after treating macrophages with H 2 O 2 , H 2 O 2 +MnO 2 , H 2 O 2 +TMNP, or H 2 O 2 +MnO 2 @TMNP. Data are presented as the mean ± SD ( n = 3): ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 between groups.

Article Snippet: The cytokines used in this study (TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)), lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress (MCE, China).

Techniques: Flow Cytometry, Fluorescence, Control